Background:
@EN The influence of the environment on a culture is expressed via four routes : (1) the nature of the substrate or phase on or in which the cells grow; (2) the physicochemical and physiological constitution of the medium; (3) the constitution of
the
gas phase; and (4) the incubation temperature. Melanization is closely related to the constitution and amounts of amino acids in the medium.
@ES Objective:
@EN The aim of this study is to investigate whether there are some differences of proliferation and melanization in cultured B16F1 mouse melanoma cells according to different culture media.
@ES Methods:
@EN We examined the color of cell pellet, cell morphology, electron microscopic findings, cell counts and melanin contents in B16F1 mouse melanoma cells cultured in Dulbecco's modified Eagle's medium(DMEM), F-10, MCDB 153, Minimal essential
medium(MEM),
and RPMI 1640, respectively.
@ES Results:
@EN 1. The color of cell pellets ranged from dark gray to light brown. The order of the darkness was DMEM, MEM, RPMI 1640, MCDB 153, and F-10 medium.
2. Most B16F1 mouse melanoma cells had an epithelioid morphology, but a few cells in MCDB 153 medium showed dendrites. On the 4th day after culture, the cells in F-10 medium were larger than those in the other media.
3. In the electron microscopic findings, B16F1 mouse melanoma cells in DMEM and MEM contained numerous stage IV melanosomes, however, those in RPMI 1640 and MCDB 153 medium contained a few, and those in F-10 medium did few.
4. The number of B16F1 mouse melanoma cells were 1.42±0.06×10E6, 1.42±0.12×10E6, 1.17±0.08×10E6, 0.73±0.06×10E6, 0.32±0.01×10E6, in RPMI 1640, DMEM, MEM, F-10, and MCDB 153 medium, respectively.
5. In the MTT assay, the order of the optical density of B16F1 mouse melanoma cells in various media was as followings; DMEM, RPMI 1640, MEM, F-10, and MCDB 153.
6. Compared with the melanin contents of B16F1 mouse melanoma cells in DMEM< they were 77.97% in MEM, 67.91% in RPMI 1640 and MCDB 153 medium, and 55.9% in F-10 medium.
@ES Conclusion:
@EN The phenotypic changes of B16F1 mouse melanoma cells were induced by various culture media and were reversible, Since the phenotypes of cells can be changed by the culture media, researchers should choose the appropriate culture medium for
the
cells. (Kor J Dermatol 1995;33(5) : 914~921)
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